KMID : 0043320120350050897
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Archives of Pharmacal Research 2012 Volume.35 No. 5 p.897 ~ p.904
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Inhibitory effects of glycyrrhizae radix and its active component, isoliquiritigenin, on A¥â(25?35)-induced neurotoxicity in cultured rat cortical neurons
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Lee Hong-Kyu
Yang Eun-Ju Kim Joo-Youn Song Kyung-Sik Seong Yeon-Hee
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Abstract
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This study investigated an ethanol extract from Glycyrrhizae radix (GR), the root of Glycyrrhiza uralensis (Leguminosae), for possible neuroprotective effects on neurotoxicity induced by amyloid ¥â protein (A¥â) (25?35) in cultured rat cortical neurons. Exposure of cultured cortical neurons to 10 ¥ìM A¥â (25?35) for 36 h induced neuronal apoptotic death. GR (10?50 ¥ìg/mL) prevented the A¥â (25?35)-induced neuronal apoptotic death, as assessed by a MTT assay and Hoechst 33342 staining. Furthermore, GR decreased the expression of Bax and active caspase-3, proapoptotic proteins, and increased Bcl-2, an antiapoptotic protein. GR also significantly inhibited A¥â (25?35)-induced elevation of the intracellular Ca2+ concentration ([Ca2+]i) and generation of reactive oxygen species (ROS) measured by fluorescent dyes. Isoliquiritigenin (1?20 ¥ìM), isolated from GR as an active component, inhibited A¥â (25?35)-induced neuronal apoptotic death, elevation of [Ca2+]i, ROS generation, and the change of apoptosis-associated proteins in cultured cortical neurons, suggesting that the neuroprotective effect of GR may be, at least partly, attributable to this compound. These results suggest that GR and isoliquiritigenin prevent A¥â (25?35)-induced neuronal apoptotic death by interfering with the increases of [Ca2+]i and ROS, and GR may have a possible therapeutic role for preventing the progression of neurodegenerative disease such as Alzheimer¡¯s disease.
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KEYWORD
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Glycyrrhizae radix, Isoliquiritigenin, Cultured neurons, Amyloid ¥â protein, Neuroprotection, Alzheimer¡¯s disease
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